Immunohistochemical study of CD74 biomarker in normal and malignant breast tissues.

OBJECTIVE: Aim: To detect the role of CD74 expression in breast carcinoma as a predictive marker for identifying the biological behavior of malignancy in Iraqi women.. PATIENTS AND METHODS: Materials and Methods: The study used technique of immunohistochemistry for detection CD74 protein role in breast cancer, and its expression in breast cancer tissue samples. Samples were collected in Al-Najaf city in Iraq, from Al-Forat Al-Awsat Oncology Center. The study was achieved at the Laboratories of the Faculty of Science in the University of Kufa. Fifty samples of breast cancer tissue, and twenty controls benign tissue were included in the study. The study has investigated relationship between expression of biomarker with grade, age of patient and tumor size. RESULTS: Results: The study showed that the cytoplasmic expression of CD74 with more clear and intensive staining in the cytoplasm, and reported that CD74 positivity rate was 52%. A significant association between CD74 expression and grade and size of tumor, so CD74 can be considered as a biomarker for prediction of breast cancer in women. No association was found between CD74 expression and each of patients' age and node metastasis. CONCLUSION: Conclusions: The study represents an important step in our region because there are a few studies about this topic; more efforts are required to approve the function of this biomarker.

Biochemical changes in patients during hypothyroid phase after thyroidectomy.

Hypothyroidism is the most common long-term consequence after total thyroidectomy. The objective of the present study was to evaluate the lipid profile and liver function in patients after hypothyroidism. Sixty patients who underwent a surgical operation to remove thyroid were included in this study, and thirty healthy subjects were used as a control. The study was conducted at Al-Sadr Medical City in Al-Najaf city, in Iraq, from October 2020 to March 2021. Thyroid-stimulating hormone (TSH) was very high in patients at a hypothyroid stage after hypothyroidism. The results showed a significant increase in total cholesterol, triglycerides (TG), low-density lipoprotein (LDL), and the ratio of total cholesterol/high-density lipoprotein (HDL). The study also revealed a significant increase in the liver enzymes aspartate aminotransferase (AST) and alanine transaminase (ALT) and a significant decrease in alkaline phosphatase (ALP) in patients with thyroidectomy compared to the control group. The correlation matrix revealed a strong positive correlation between TSH levels and total cholesterol, triglycerides, LDL, AST, ALT, and ALP. It was concluded that hypothyroidism, the major consequence of thyroidectomy, causes dysfunction in lipid metabolism and liver enzymes resulting in secondary hyperlipidemia and liver dysfunction.